Moreover,cell adsorption occurs in reaction media. The attachment of bacterial cells to the surface of a support matrix is happens due to attraction between cells and matrix. The affinity of different cell types to the support matrix primarily depends on the age of cells and their chemical nature. It is an immobilization technique whereby cells adhere to the surface of an inert support. Gel entrapped cells are useful at industrial scale for the production of hydroquinone, L-malic acid, amino acids such as isoleucine and L-aspartic acid.įor instance, mixing of Alcaligenesspecie’s cell suspension with 4% sodium alginate solution in 1:2 ratio followed by the extrusion of this mixture into CaCl 2 solution by means of a bead forimg device gives alginate beads containing immobilized cells for industrial use. Once cells are loaded into the gel or the polymeric solution, the combination is then extruded through the needle to allow it to harden and form beads. This methodology additionally requires introduction of cross linking agents to strengthen the cell trap. This is the most safest and easy to conduct method of cell immobilization which involves usage of polymeric networks such as synthetic or natural polymers to entrap cells and immobilize them. Various cell immobilization techniques 1. One of the problem plant cells immobilization is that the product may entrap in cell vacuole which affects the total quantity of the product manufactured.Ĭell immobilization of bacterial cells is also useful for industial biochemical processes. The engineers focus to improve the designs of bioreactors to reduce cost and to maximize the rate of production. Therefore, cell immobilization technique for biochemical processes still needs improvement.
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